MALDI-TOF-MS/HPLC-UV-VIS rHGH results

[ProfessorX]

But you must have GH in your blood(serum) before it can convert to IGF-1. I have mentioned this before and everyone keeps missing this step. A serum GH is nothing more than how much GH is in your blood. The more GH you have in your blood from an injection, the more that will be converted to IGF-1

So......IGF1 is used to monitor GH Therapy. The therapeutic effects of GH are mediated by IGF1

THE METABOLIC ACTIVITY OF GH WILL OUTLAST THE ACTUAL GH LEVELS IN THE BODY

 

[muscle96ss]

So......IGF1 is used to monitor GH Therapy. The therapeutic effects of GH are mediated by IGF1

THE METABOLIC ACTIVITY OF GH WILL OUTLAST THE ACTUAL GH LEVELS IN THE BODY

Actually, not all the therapeutic effects are mediated by IGF-1, but most yes.

But in respone to your statement, IGF-1 is used to monitor GH therapy because the doctor is not interested in determining how much GH you took; he is interested in determining how your body processed that GH. As I explained earlier, he is interested in the end product and could care less about the means. If you are looking to determine what your bodies reaction is to your GH, then an IGF-1 is by far the best way to go. In fact a lab test won't even tell you that(although the lab test can tell you other important information like toxins and impurities,etc..).

 

[ProfessorX]

IGF1:
The spurious relationship between serum IGF1 levels and growth response precludes the use of IGF1 as a surrogate marker for efficacy.

HPLC:
Identifies rhGh Somatropin (191aa)
Purity %
Mg/IU

GH Serum:
GH Protein Present

LabCorp Identifies
22k + (proteins greater) 191aa
20k + (proteins greater) 176aa

LabCorp does not only identify Somatropin rhGh:

r-hGh - 191aa
Met-hGh - 192aa
des-Phe1-hGH - 190aa

I'm glad we agree.

 

[ProfessorX]

Also, one last thing. All the IGF-1 tests that we have been performing via Labcorp are done via ICMA and not by RIA. RIA is the gold standard and more expensive. ICMA is prone to error and not that reliable

I honestly don't known much about the LabCorp testing methods. Only that I have seen different methods listed on my own personal blood work.

Acromegaly : (IGF1 Serum, GH Serum)(LabCorp)

Circulating human growth hormone (GH) consists of several molecular isoforms. Increased proportion of circulating non-22K hGH and 20K hGH was reported in active acromegaly

The diagnosis and treatment decisions in acromegaly depend on the measurement of growth hormone (GH) and insulin-like growth factor I (IGF-I) levels. The occurrence of different GH isoforms in the serum, mainly 22K-hGH and 20K-hGH, is a source of heterogeneity of GH results measured by different immunoassays.

Since GH Serums are a big part of your testing, maybe look into exactly what the test is testing for. (22k+ / 20k+) (LabCorp serum Vs HPLC vial)

Does the Testosterone Serum test distinguish between Test P, Test E, Test C? Or will they all elevate the Testosterone Serum.

 

[muscle96ss]

Does the Testosterone Serum test distinguish between Test P, Test E, Test C? Or will they all elevate the Testosterone Serum.

They will all elevate serum T levels.

 

[muscle96ss]

I honestly don't known much about the LabCorp testing methods. Only that I have seen different methods listed on my own personal blood work.

Acromegaly : (IGF1 Serum, GH Serum)(LabCorp)

Circulating human growth hormone (GH) consists of several molecular isoforms. Increased proportion of circulating non-22K hGH and 20K hGH was reported in active acromegaly

The diagnosis and treatment decisions in acromegaly depend on the measurement of growth hormone (GH) and insulin-like growth factor I (IGF-I) levels. The occurrence of different GH isoforms in the serum, mainly 22K-hGH and 20K-hGH, is a source of heterogeneity of GH results measured by different immunoassays.

Since GH Serums are a big part of your testing, maybe look into exactly what the test is testing for. (22k+ / 20k+) (LabCorp serum Vs HPLC vial)

Labcorp tests only for the 22K form of HGH in the serum test.

 

[ProfessorX]

So how is Acromegaly tested accurately? (GH Serum / IGF1 Serum)

Growth hormone (GH) is a 191aa heterogeneous peptide produced by pituitary cells. GH comes is a variety isoforms including: a 22 kDa isoform / a 20 kDa isoform and several post-translationally modified GH forms.

The International Standard 80/505 (20kDa and 22kDa)

The International Standard 98/574 (22kDa)

 

[ProfessorX]

Most currently used assays are calibrated against the pituitary-derived International Standard (IS) 80/505 (20kDa and 22kDa) from the WHO.

In addition to the differences in calibration, there were larger differences in GH values at low concentrations. The Orion RIA did not measure concentrations 1 to 2 g/L; thus, it was not useful for measurement of nadir GH values during an OGTT. The difference in values mea- sured by the 2 immunometric assays also increased at low values. At these concentrations, the difference was 2- fold.

In conclusion we found that various GH assays have different results

Dr A from SIMEC explained to me VIA email the complexity of testing rhGh compared to AAS (APIs /Finished product)

I would imagine blood serum to be more complex

I just can't see relying on those GH Serums other than GH Protein present

"They will all elevate serum T levels"

The same as all GH isoforms will elevate GH Serums

 

[muscle96ss]

Most currently used assays are calibrated against the pituitary-derived International Standard (IS) 80/505 (20kDa and 22kDa) from the WHO.

In addition to the differences in calibration, there were larger differences in GH values at low concentrations. The Orion RIA did not measure concentrations 1 to 2 g/L; thus, it was not useful for measurement of nadir GH values during an OGTT. The difference in values mea- sured by the 2 immunometric assays also increased at low values. At these concentrations, the difference was 2- fold.

In conclusion we found that various GH assays have different results

Dr A from SIMEC explained to me VIA email the complexity of testing rhGh compared to AAS (APIs /Finished product)

I would imagine blood serum to be more complex

I just can't see relying on those GH Serums other than GH Protein present

"They will all elevate serum T levels"

The same as all GH isoforms will elevate GH Serums

Endogenous GH does have several isoforms which makes testing for all isoforms complex.

However, exogenous GH is solely in the 22KDa form and therefore should not be complex at all. So your last statement that all GH isoforms will elevate GH serums is incorrect for at least the labcorp test as they test specifically for 22KDa isoform.

Finally, Dr. A is correct, GH testing is a whole different beast than AAS testing.

 

[ProfessorX]

I see where your confusion is now

So all the variations (20kDa - 22kDa+) will elevate the basic $50 LabCorp GH Serum test

That's the potential issue with unregulated Generic GH

HPLC will target SOMATROPIN 191 aa

"exogenous GH is solely in the 22KDa form"

If it's a regulated approved GH

Generics are a toss of the dice

Lots of GH variations will always elevate GH Serums


Example of Diminishing IGF1

Dimmer and related substances of higher molecular mass: rhGh aggregates that coexist with normal rhGh. They don't have biological activity and cause antibody of rhGh. The antibody combines to rhGh molecule. The antibody prevents the rhGh from combining to it's receptor to stimulate growth, cause the biological activity of rhGh to decrease

 

[muscle96ss]

View attachment 33231

I see where your confusion is now

So all the variations (20kDa - 22kDa+) will elevate the basic $50 LabCorp GH Serum test

That's the potential issue with unregulated Generic GH

HPLC will target SOMATROPIN 191 aa

"exogenous GH is solely in the 22KDa form"

If it's a regulated approved GH

Generics are a toss of the dice

What are you talking about? Did you read what I wrote, the labcorp is 100% specific to the 22kDA isoform, so no it will not pick up the others. And that is a negative aspect of the test, not something positive as you are portraying it as technically in normal cases you want a test that can pick up all the GH in your blood. Although it is a positive aspect in our case where what we are injecting is only going to be the 22kda variety.

So much misinformation and so much paranoia. Prof X, I sure hope you are this diligent about the food you put in your body(not to mention the chemicals you put on your body or are exposed to throughout life). Who knows where it has come from, what it contains, what type of genetic modifications it had undergone, etc...

 

[ProfessorX]

What are you talking about? Did you read what I wrote, the labcorp is 100% specific to the 22kDA isoform, so no it will not pick up the others. And that is a negative aspect of the test, not something positive as you are portraying it as technically in normal cases you want a test that can pick up all the GH in your blood. Although it is a positive aspect in our case where what we are injecting is only going to be the 22kda variety.

So much misinformation and so much paranoia. Prof X, I sure hope you are this diligent about the food you put in your body(not to mention the chemicals you put on your body or are exposed to throughout life). Who knows where it has come from, what it contains, what type of genetic modifications it had undergone, etc...

Most currently used assays are calibrated against the pituitary-derived International Standard (IS) 80/505 (20kDa and 22kDa) from the WHO.

It's just info buddy

There's some really good Generic GH out there, no doubt

But not all of it...the simple GH Serum test does not show either way

Nor does it determine "overdosed"

 

[ProfessorX]

LabCorp:


Growth hormone (GH) testing is primarily used to identify growth hormone deficiency and to help evaluate pituitary gland function, usually as a follow-up to other abnormal pituitary hormone test results.

GH testing is also used to detect excess GH and to help diagnose and monitor the treatment of acromegaly and gigantism.

Acromegaly : (IGF1 Serum, GH Serum)(LabCorp)

Circulating human growth hormone (GH) consists of several molecular isoforms. Increased proportion of circulating non-22K hGH and 20K hGH was reported in active acromegaly

The diagnosis and treatment decisions in acromegaly depend on the measurement of growth hormone (GH) and insulin-like growth factor I (IGF-I) levels. The occurrence of different GH isoforms in the serum, mainly 22K-hGH and 20K-hGH, is a source of heterogeneity of GH results measured by different immunoassays.

GH Serum is not a HPLC Lab Analysis

 

[muscle96ss]

LabCorp:


Growth hormone (GH) testing is primarily used to identify growth hormone deficiency and to help evaluate pituitary gland function, usually as a follow-up to other abnormal pituitary hormone test results.

GH testing is also used to detect excess GH and to help diagnose and monitor the treatment of acromegaly and gigantism.

Acromegaly : (IGF1 Serum, GH Serum)(LabCorp)

Circulating human growth hormone (GH) consists of several molecular isoforms. Increased proportion of circulating non-22K hGH and 20K hGH was reported in active acromegaly

The diagnosis and treatment decisions in acromegaly depend on the measurement of growth hormone (GH) and insulin-like growth factor I (IGF-I) levels. The occurrence of different GH isoforms in the serum, mainly 22K-hGH and 20K-hGH, is a source of heterogeneity of GH results measured by different immunoassays.

GH Serum is not a HPLC Lab Analysis

Here is a copy and paste from the labcorp website. Pay careful attention to the last paragraph where is states that the assay is calibrated to the WHO standard listed and 100% specific to the 22kda isoform:

Growth Hormone
Synonyms:

• Human Growth Hormone (hGH)
• Somatotropic Hormone (STH)

Test Number: 004275 CPT: 83003 (per specimen)
Related Information

• Growth Hormone Stimulation
• Growth Hormone Suppression Test for Acromegaly
• Multiple-specimen Testing

Specimen
Serum

Volume
0.8 mL

Minimum Volume
0.3 mL (Note: This volume does not allow for repeat testing.)

Container
Red-top tube or gel-barrier tube

Collection
If a red-top tube is used, transfer separated serum to a plastic transport tube. Label tube with time of collection and patient's name.

Storage Instructions
Refrigerate

Stability
Temperature Period
Room temperature 7 days
Refrigerated 14 days
Frozen 14 days
Freeze/thaw cycles Stable x3
Patient Preparation
Random growth hormone sampling should be performed on fasting patients who have rested for at least 30 minutes prior to collection. Growth hormone stimulation and suppression protocols are described in the online Endocrine Appendices: Growth Hormone Stimulation and Growth Hormone Suppression.

Causes for Rejection
Gross hemolysis; lipemia; plasma specimen

Reference Interval
0.0−10.0 ng/mL

Use
Pituitary function test useful in the diagnosis of hypothalamic disorder, hypopituitarism, acromegaly, and ectopic growth hormone production by neoplasm

Limitations
A single fasting growth hormone (GH) level is of limited value. Secretion of GH is episodic and pulsatile. GH has a half-life of 20 to 25 minutes. Testing for growth hormone deficiency or excess is best done as part of a dynamic test involving specific stimuli (see the online Endocrine Appendices: Growth Hormone Stimulation and Growth Hormone Suppression). Insulin-like growth factor-1 can also be useful in assessing growth hormone status.

As in the case of any diagnostic procedure, results must be interpreted in conjunction with the patient's clinical presentation and other information available to the physician.

Methodology
Immunochemiluminometric assay (ICMA)

Additional Information
Human growth hormone (hGH) is a polypeptide hormone secreted from the acidophil cells of the anterior pituitary gland. Secretion is episodic and is associated with exercise, the onset of deep sleep or postprandially in response to falling glucose levels. Synthesis and release are under the control of hypothalamic releasing peptides and inhibitory peptides such as somatostatin. More recently, a gastric peptide, ghrelin, has been shown to also stimulate HGH secretion. The mediator of many hGH actions in the periphery, insulin-like growth-factor I (IGF-I) exerts an inhibitory effect through negative feedback mechanisms.1 hGH in circulation consists of several molecular isoforms, with 22,000 Dalton hGH being the most abundant, followed by a 20,000 Dalton hGH variant produced by alternative splicing. Approximately 50% of circulating hGH is bound to a high affinity binding protein.2 hGH is physiologically important in two main areas. Firstly, it has an integral role in skeletal growth which is well demonstrated in either excess or deficiency in childhood. The action of hGH in part is mediated through IGF-I as well as promoting protein synthesis and the uptake of amino acids into cells. Secondly, hGH influences intermediary metabolism by stimulating lipolysis and is antagonistic to the insulin-mediated uptake of glucose.3 hGH secretion is stimulated by hypoglycemia and suppressed by hyperglycemia.

In childhood, symptoms of hGH deficiency are retarded growth and dwarfism. Etiology is often unknown and an absolute or relative deficiency usually becomes apparent at about two years of age. Diagnosis can be confirmed by demonstrating low serum hGH which does not respond to stimulation tests. hGH deficiency is a major cause of severe short stature and diagnosis at an early stage is essential for successful therapy.4 Hyposecretion in adults usually becomes apparent during the laboratory investigation of hypopituitarism.5,6

Hypersecretion, commonly due to adenoma of the acidophil cells, is characterized by two conditions depending on whether it becomes apparent before or after fusion of the bony epiphyses. In childhood, excess hGH is characterized by gigantism. Heights of eight feet may be achieved and may also be associated with hypogonadism. In adults, acromegaly results, a condition characterized by progressive thickening of bone and soft tissue. Diagnosis is usually confirmed by dynamic function testing, which demonstrates a raised serum hGH level that does not fall in response to an oral glucose load.7 In conditions where there are nutritional disturbances, such as anorexia, starvation, renal failure, and hepatic cirrhosis, increased basal hGH levels may be found.

Recombinant hGH is available for treatment of hGH deficiency in both children and adults.4-6 hGH excess is treated by surgery, irradiation therapy, or somatostatin analogues.8,9 More recently, pegvisomant, a hGH receptor antagonist, which shares structural homology to hGH and competes with hGH for binding to the hGH receptor, has been developed.10

The IDS iSYS hGH assay conforms to the recommendations outlined in the recently published consensus statement on the standardization and evaluation of growth hormone assays.11 The assay is calibrated to the WHO International Standard for Somatropin from NIBSC, code 98/574.12 The assay is 100% specific for the 22 kDalton form of hGH and has no cross-reactivity with pegvisomant.13

 

[muscle96ss]

There's some really good Generic GH out there, no doubt

But not all of it...the simple GH Serum test does not show either way

Nor does it determine "overdosed"

As both buck and I explained yesterday, a 15iu sample serum tested in the exact same manner by the same tester as a 10iu sample, should score higher than the 10iu. Why? Because there is more GH in the serum when you inject 15iu compared to 10iu; not too hard to understand. So, if a 10iu sample is overdosed to 15iu(as TP's new 5mg black tops that were lab tested at 5mg are); then the serum results should be higher when testing this product. Guess what?? People are getting really high serums on the new 5mg black tops!! Shocking! But it is most likely coincidence as serums have nothing to do with dosage.

 

[ProfessorX]

muscle96ss said: ↑
Also, one last thing. All the igf-1 tests that we have been performing via Labcorp are done via ICMA and not by RIA. RIA is the gold standard and more expensive. ICMA is prone to error and not that reliable

Methodology
Immunochemiluminometric assay (ICMA)

code 98/574.12 The assay is 100% specific for the 22 kDalton form of hGH and has no cross-reactivity

Orion RIA
Most currently used assays are calibrated against the pituitary-derived International Standard (IS) 80/505 (20kDa and 22kDa) from the WHO.

In conclusion we found that various GH assays have different results

 

[ProfessorX]

So, if a 10iu sample is overdosed to 15iu(as TP's new 5mg black tops that were lab tested at 5mg are); then the serum results should be higher when testing this product. Guess what?? People are getting really high serums on the new 5mg black tops!! Shocking! But it is most likely coincidence as serums have nothing to do with dosage.

So these were Tested to see 5mg?

Hopefull the new samples come back soon

We can analyze the results together when they are done. I'm dealing with Matthias also....so I'm guessing after the 7th

Hopefully it'll shed some light on the subject.....or maybe just confuse us more !

 

[muscle96ss]

muscle96ss said: ↑
Also, one last thing. All the igf-1 tests that we have been performing via Labcorp are done via ICMA and not by RIA. RIA is the gold standard and more expensive. ICMA is prone to error and not that reliable

Methodology
Immunochemiluminometric assay (ICMA)

code 98/574.12 The assay is 100% specific for the 22 kDalton form of hGH and has no cross-reactivity

Orion RIA
Most currently used assays are calibrated against the pituitary-derived International Standard (IS) 80/505 (20kDa and 22kDa) from the WHO.

In conclusion we found that various GH assays have different results

Yes, RIA is more expensive and more reliable!!

 

[muscle96ss]

Prof - X: buck found a gem last night. I believe it was you(may have been rpbb or both) that posted a link to a Dr. Elmer Cranton to back up some of your points against serum testing. Well here is what that same doctor has to say about IGF-1 testing, it seems as if he is saying some of the same things that I have been saying. I have copied and pasted it, but here is the link as well since the layout of the tables is messed up when I paste it here; so its much easier to read at the link. Anyway, figured you would enjoy it.

Interpretation of IGF-1 Lab Tests, and IGF-1 and prostate cancer



Welcome to Ellis Toussier-Ades Bigio-Antebi's


IGF-1 Interpretation
and

IGF-1 Laboratory Tests

and

Correlation Between IGF-1 and Prostate Cancer



and

Dr. Cranton Replies to Questions Re: rHGH Growth Hormone

by


Elmer Cranton, M.D.
The following was posted by Dr. Elmer Cranton on the Rejuvenation newsgroup, which is for persons taking human growth hormone (HGH) by sub-cutaneous injection, or persons interested in following the discussion. I copy these posts here with Dr. Cranton's permission, because of their value for others to read. -Ellis Toussier

From Elmer Cranton, M.D.
Re: IGF-1 interpretation

The following must be considered when relying on IGF-1 reports:

1. I have found that laboratories are not always accurate in testing IGF-1 (sometimes called somatomedin-C).Different methods are used by different labs and the results are not always comparable. It is a mistake to believe that clinical laboratory reports are always reliable. IGF-1 is a very specialized test. Unless a lab is large enough to have a substantial volume for that special test, calibration and standardization may not be accurate. I have found that Smith Kline Lab, LabCorp Lab, and King James OmegaTech Lab are the most reliable. That doesn't mean that others may not also be good. But be suspicious of reports if they don't make sense clinically.

2. IGF-1 is not HGH It is a metabolic breakdown product made from HGH by the liver. IGF-1 has some hormone activity by itself but HGH in its pure form has much broader activity. Because HGH remains in the blood for only a few minutes before attaching to cell receptors, IGF-1 is used as an easily obtained but partial indicator. IGF-1 stays in the blood for a day or more. IGF-1 seems to be reliable as a guideline for internal pituitary production, which is pulsitile over many hours. But people differ widely in the amount of IGF-1 produced from HGH by injection. I have seen patients with only mild increases in IGF-1 from injected HGH respond quite dramatically, out of proportion to IGF-1 figures.

3. The fact is that1 unit of HGH equals the total daily pituitary production for a healthy young adult. Therefore one unit of HGH daily is a total replacement dose in old age, regardless of the IGF-1 followup. It is possible that more of the HGH taken by injection (once or at most twice daily) attaches to cells receptors in the body, perhaps more effective ones, and that less goes to the liver to be broken down to IGF-1.

4. I do not do routine follow-up IGF-1 blood tests in my patients for the above reasons. Those measurements have not correlated with replacement doses and vary widely from person to person. There is a wide variation in how much IGF-1 increases from person to person. This seems to have no significance to long-term benefit seen in my clinical practice.

THE DEGREE OF INCREASE IN IGF-1 DOES NOT SEEM TO CORRELATE WITH CLINICAL BENEFIT. BUT THE LEVEL OF BASELINE IGF-1, BEFORE INJECTIONS BEGIN, AS A PRODUCT OF SLOW AND CONTINUOUS PITUITARY RELEASE, DOES SEEM TO BE A RELIABLE INDICATOR OF HOW DEFICIENT A PERSON IS TO BEGIN WITH.

5. I know that for myself personally, each unit of HGH by injection raises my IGF-1 by 100 nanograms/milliliter (ng/ml). That is my own consistent measurement if the HGH is real and not counterfeit. Someone else may have a different reading, more or less, with no significance to benefit. I am 67 years old and my baseline IGF-1 without replacement is approximately 100. Every time I get a new lot number of HGH for my patients I take one unit daily for one week and test my IGF-1. It should be about 200. I then take 2 units daily for several days and test again. It should be about 300.

That is the only way I can be sure of getting the real thing. Over the past 2 years I have twice received counterfeit HGH, properly labeled and otherwise indistinguishable from the real thing. When I tested my IGF-1, it remained at baseline.

I now use Lilly Humatrope in my practice. It has a foreign label but is made in France in the same factory as the USA product. It has consistently been the best in my own testing. IGF-1 measurements will also vary by 10% to 20% from day to day normally. And if a single blood specimen is split into two test tubes and sent to the same laboratory, the results may differ by 20%. The test method is only that accurate. Variations between different labs may be even greater. That fact must be considered in interpretation.

6. HGH replacement therapy means replacement therapy for deficiency with aging. Young people produce plenty of their own and young adults also respond much more briskly to precursors (various amino acids). When reading claims for precursors of any type, it is necessary to know if IGF-1 was deficient to begin with. I would ask to see results for a series of 10 patients over 70 years old (whose IGF-1 will be around 100, plus or minus) and then get before and after readings. They must be 10 sequential patients, not the best 10 responders out of 100, as may be deceptively done. On the average I have only seen about 25% increase of IGF-1 with amino acids in such patients. And to get that increase it is necessary to have an empty stomach, no food for several hours before and 2 hours after taking that product. Food competes with the amino acids for absorption. It is necessary for amino acids to go in fast without interference from other foods to boost HGH release. Young people who do not need HGH and who will not benefit from more will increase much more with the amino acids than old people who really need it.

7. It is well known that HGH releasers (peptides, amino acids, releasing hormone, etc.) lose there effect over time. The pituitary becomes tolerant to them and releases less and less HGH over several months. They work best short term, best in young people who don't need the benefit and lose their effect with time.

Elmer M. Cranton, M.D.

> From: "Dale R. & Karen A. Hersh"
> Dear Dr. Cranton,
> Thank you so much for you information, but I do have one question reference point 5: Do you regularly use rHGH and if you don't why? -- And if you do -- do you cycle it?

I regularly take one to two units of rHGH daily, every day. I've done that for 3 years now with good results. I do not cycle it.

Cycling can save money and get more bang for the buck, but has reduced overall benefit.

E M Cranton, MD

From: "Elmer Cranton, M.D."

Subject: IGF-1 Laboratory Tests

I did a comparison study of IGF-1 test results by sending split specimens to several large, very reputable reference laboratories.

I took a single blood specimen, I split it into two tubes and sent them to two different laboratories. Theoretically the results should have been the same. There was a correlation but also large differences.

The results follow:

The two test results are on the same, identical blood specimens.

The laboratories are large, highly reputable, fully licensed labs, approved, and regularly inspected by the government. This cost me some money as each test cost more than $60. But I felt it was necessary to correctly interpret my patients' results.

When you see IGF-1 figures used in marketing, you should keep the below results in mind. Any such figures mean nothing unless the standard deviation of the method used is known and enough tests are done to get a statistically significant difference. A you can see, differences of 25% to 50% are within the error of the method and can be quite meaningless.

It is also easy for a marketer to select the most favorable numbers and discard those that do not favor a product. How can one be sure this is not done? Unless the research is done independently, by a researcher with nothing to gain or lose form the results, such studies are always suspect in my mind. If a marketing company pays for research, will the researcher bite the hand that feeds him/her???

IGF-1 on same specimen, ng/ml



LAB A

LAB B

140

186

236

301

97

124

124

125

98

131

215

284

250

406

97

124

126

169

67

89

180

331

200

379

66

133

261

143

424

546

103

159

175

215

So I repeated the test with another two labs, to see if it was just the above two labs or a consistent problem.








LAB C

LAB D

177

174

165

84

255

381

222

208

57

24

132

60

133

31

I am now repeating this test by sending samples of the same blood to the same lab on different weeks to see the differences in results using the same lab both times.

So far the results are similar to the above, although the differences are less.

Elmer M. Cranton, M.D.




Dale comments on the above results:
From: "Dale R. & Karen A. Hersh"
Subject: Re: IGF-1 Laboratory Tests
At first I looked at this as though these results show that testing IGF-1 was probably useless and this bothered me. Then I started looking at them a little closer and saw quite a bit of consistency. Lab B was mostly higher than A and when I put a calculator to the results, 10 of the split samples differ the same ratio .77 +/- .03

4 more had similar ratios, but at .55

Actually, these are pretty consistent results. It seems like it's more a question of calibration (if there is such a thing for IGF) or even who ran the test.

Depending on what days these results were run, there is more consistency than appears to be the case at first glance... A few were completely off and I can't explain that without more information. So using the same lab may be the answer if one is going to get their IGF tested.

I couldn't see any correlation between C and D.


Correlation of IGF-1 and Prostate Cancer
A good study has shown that a large number of elderly male patients taking HGH over a long time had no increase in prostate cancer.

That rumor got started when it was reported that elderly men with the lowest quintile of IGF- (lowest fifth of HGH) had less cancer than men with the highest 20% (highest fifth). There was no linear correlation of IGF-1 and prostate cancer in the study. They only reported less cancer in the most deficient of an elderly population.

What that report did not point out was that the lowest fifth were so deficient that all tissues of the body were inhibited in growth, healing and maintenance-- healthy as well as cancerous. It is quite a different thing to state that deficiency of essential hormones slows the growth of cancer (and everything else including a healthy body) and, on the other hand, trying to prove that hormones cause cancer. Life and health cannot progress without hormones. Cancer cannot progress without a living body to support its growth. It is quite predictable that if old people are dying from end stage deficiencies, they might have less cancer.

If all of the facts were reported, it is my opinion that the most senile and debilitated of the group would also have been those in the lowest 20% of IGF-1. But that data was not presented.

If HGH is only replaced to an average level present in the body for 30 years, from age 20 to age 50, and if it was safe during that 30 year period, and if it was essential to health during those 30 years, what is the harm in replacing it after age 50 when it becomes deficient? If it's dangerous, why does it not cause problems in the earlier 30 years when it is normally present in the same levels or higher from pituitary production? (Note: Excessively high doses of hormones can be harmful, I am referring here only to normal replacement doses).

E M CRANTON, MD





For more information on Dr. Elmer Cranton's EDTA Chelation Therapy or growth hormone replacement therapy, go to:www.drcranton.com.

Dr. Cranton's Replies Answers Questions referring to Growth Hormone (rHGH) Replacement Therapy!

Dr. Cranton Re: Growth Hormone Replacement



This and other posts by Dr. Cranton to the Rejuvenation board are found in Digest No. 26, 27, and 28, which are found in the Archives of the
http://groups.yahoo.com/subscribe/Rejuvenation









________________________________




I invite you to http://groups.yahoo.com/subscribe/Rejuvenation... Many say it is the best anti-aging forum on the internet. Rejuvenation has more than 2000 anti-aging doctors and patients subscribed. There are more than 10,000 questions referring to the use of authentic injectible growth hormone and other anti-aging therapies in the Archives of Rejuvenation, most of them with answers annotated on the post.








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