Thats how i add a little bit of christmas spirit in every vial. Pink tutu and balerina shoes.
MESO-Rx
Anabolic Steroids
PurplePandaLabs Raw source
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colossus25
Member
Lmaoo that'll put some tren in your stockings [emoji23]
TRT
New Member
I can respect that. We've all said some incriminating shit. Lol I know I have.
Mr_Nobody
New Member
I can get a full report tomorrow and show everybody here, what it is suppose to look like... Just know that it will not be for amino acid sequencing (roids)
But the data should still be the same for gsmc , concerning how the software for any lab will have a library that you can identify the substances in a sample even if they are unknown. Just take a little more work.
But the data should still be the same for gsmc , concerning how the software for any lab will have a library that you can identify the substances in a sample even if they are unknown. Just take a little more work.
TRT
New Member
Please do.
janoshik
Subscriber
No Sir, the solvents job is to dissolve the sample and elute it, separation is done by stationary phase on the column.
I completely agree that textbook and real world experience are not the same, the books can only get you so far - I've conducted over 1000 analyses since 2016.
No, that is a full result sheet from a chromatographic run.
You graph for ease of interpretation and each peak with retention time and area of the peak. That's literally everything you need.
After that, you usually test the standard and you use retention time (RT) in these graphs for identification and area for quantification (getting mg/IUs). However, if there is no standard to test, you'll get exactly that.
And analyzer didn't use LC/MS for HGH and I'd rather not be getting into that too much.
janoshik
Subscriber
No, you cannot compare HPLC with UV detection with GCMS, where the detection method is mass spectrometry... http://lab-training.com/landing/free-hplc-training-programme-8/ (Laboratory training courses on HPLC, GC, AAS, Lab Safety, Spectroscopy)
Please, Sir, before you spread further misinformation, educate yourself.
Having a family member working in a laboratory in no replacement of formal education or years of experience first hand.
Mayne
Well-known Member
A friend of mine is on the cialis 15mg a day and is impressed, 3/5 products have now checked as they should, anavar and stanozolol still to look at
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Mayne
Well-known Member
Anyone has experience with PPL CJC 1295 DAC I am keen on adding that with my next order so I can check how much benefit it has to add to MK I am already on
Mr_Nobody
New Member
Did you want some lube before I start??.....
so your telling me if somebody has a military parent that the child isn't going to known a shit load more than a civilian regarding all things military? or a sports athlete child won't know all the in's and outs even though they might hate sports??
Iv'e made the the key parts you didn't read so that you can see better , I have also highlighted the comma red so that you can see that when I said the data should still be the same. I was talking about how every software gas a library/index of chemicals that you can look up for an unknown substance, with a little work it's name can be found by highlighting the peak in question and breaking it down from the possibilities.
Look at the area % column.
0.06
98.05
0.18
0.41
1.31
= 100.01
that isn't a perfect 100% because of the rounding that took place .
if you take area peak of which ever peak you want you can see that this formula, (I will do all the peaks one at a time so you can see starting with area 4298 in that blacktop hgh sample information picture above.)
That should tell you that area % does not mean purity, but just how much of each compounds you have in that sample.
Now that I'm done with you, would you like a cigarette?
@TRT
after the graph report you will have data on all the internal references that you have calibrated the machine to recognize: once you get a hit for that chemical it creats a peak.
now for this test that was run the machine was calibrated for 60 specific compounds, the graph shows 9 named compounds and many more unamed peaks which are compounds not within the 60 compounds the machine is calibrated for. so if you look at the quantitation report picture, the internal standards and the surrogate compound are injected through a copper column into the sample, now the target compounds column detected 5 compounds that were found to be of the 60 compounds the machine is calibrated for. All the other spikes/peaks/hits seen on the graph are compounds that are not of the 60 and to find out there names would require you to select the peak in question and hit library search. Then it would give you a list depending how big your library/index is; could be 50,000 or 100,000 or more compounds in the index. the unknowns can be found just takes time.
Now we know where jano learned his stuff guys..... (don't take that to seriously, it was just a little jab)
So you work for shimadzu? or some company in japan?
so your telling me if somebody has a military parent that the child isn't going to known a shit load more than a civilian regarding all things military? or a sports athlete child won't know all the in's and outs even though they might hate sports??
Iv'e made the the key parts you didn't read so that you can see better , I have also highlighted the comma red so that you can see that when I said the data should still be the same. I was talking about how every software gas a library/index of chemicals that you can look up for an unknown substance, with a little work it's name can be found by highlighting the peak in question and breaking it down from the possibilities.
Look at the area % column.
0.06
98.05
0.18
0.41
1.31
= 100.01
that isn't a perfect 100% because of the rounding that took place .
if you take area peak of which ever peak you want you can see that this formula, (I will do all the peaks one at a time so you can see starting with area 4298 in that blacktop hgh sample information picture above.)
That should tell you that area % does not mean purity, but just how much of each compounds you have in that sample.
Now that I'm done with you, would you like a cigarette?
@TRT
after the graph report you will have data on all the internal references that you have calibrated the machine to recognize: once you get a hit for that chemical it creats a peak.
now for this test that was run the machine was calibrated for 60 specific compounds, the graph shows 9 named compounds and many more unamed peaks which are compounds not within the 60 compounds the machine is calibrated for. so if you look at the quantitation report picture, the internal standards and the surrogate compound are injected through a copper column into the sample, now the target compounds column detected 5 compounds that were found to be of the 60 compounds the machine is calibrated for. All the other spikes/peaks/hits seen on the graph are compounds that are not of the 60 and to find out there names would require you to select the peak in question and hit library search. Then it would give you a list depending how big your library/index is; could be 50,000 or 100,000 or more compounds in the index. the unknowns can be found just takes time.
Johne71
Member
You didn't learn this much from a family member that worked in the field. I literally worked a few feet away from an entire analytical array of equipment and watched 100s of my own samples processes daily. I don't know dick about HPLC, FTIR, MS, GC, etc...
I'm not claiming you don't know, but I'd be sure surprised if you learned through osmosis.
Nice debate we (you and Jano) have going back and forth however.
J
XmadXscientist
Well-known Member
Just out of curiosity what kind of lab did you say your family member worked in? You seem to have good knowledge of this subject.
TRT
New Member
@Mr_Nobody good shit brother. Lmao! I love it.
Think you could borrow that machine for a little? Lol
Think you could borrow that machine for a little? Lol
colossus25
Member
No you're right he uses gc/ms to test hgh, you sound critical of this. What testing method do you use?
janoshik
Subscriber
No, he didn't use GC/MS to test HGH, check your facts, please.
It's impossible to test HGH with GC/MS.
You might have LC/MS in mind and even then it's incorrect - MALDI TOF is not coupled with LC.
I use RP-HPLC and SEC-HPLC.
colossus25
Member
Ok fair enough, i know he uses gc/ms i think it was for aas i believe. Like i mentioned before my knowledge is limited as far as lab testing goes.
janoshik
Subscriber
Yeah, for AAS GC/MS is fantastic
Jeffg353
New Member
http://pubs.acs.org/doi/abs/10.1021/pr3003326
What do u think about this AAA-MS method of testing? I don't know shit about testing peptides/proteins, just something I came across doing research.
janoshik
Subscriber
Its benefits are speed, sensitivity and much easier sample prep compared to "normal" AAA, however the same limitations remain.
Peptide mapping, multiple MS structure elucidation and Edman degradation are the methods of choice of determining the structure of protein you don't have a standard of.
If you have a standard, the situation becomes much easier and the methods directly above become a brutal overkill.
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