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Did you want some lube before I start??.....

You seem hurt.

I've ignored the obviously stupid parts of your post and that doesn't leave me with too much to reply to, so I apologize about my post being much shorter than yours. You've obviously put quite a lot of time into that.

Your little maths part I can't really understand, because, well, it makes no sense, but right afterwards you are comparing HPLC with UV detection with GC/MS.

So you are comparing high pressure LIQUID chromatography with detection method consisting of uv light passing through a sample with GAS chromatography with detection method consisting of turning the sample into ions which are consequently sorted according to their mass to charge ratio.

Does that sound like the same thing to you?

so your telling me if somebody has a military parent that the child isn't going to known a shit load more than a civilian regarding all things military? or a sports athlete child won't know all the in's and outs even though they might hate sports??
I'm saying that the kid would get slapped like a bitch if the kid thought he can shittalk a real army officer. A wonderful parallel, you should take it by the heart.
 
Never used anavar before this but 30-40mg per day.. I'm cutting and getting stronger and pumps are insane veins popping out on triceps.... So Im ordering more soon.
I have some that I got with my last order. Think I'm going to use it towards the end of my cycle. Glad to hear you like it.
 
You didn't learn this much from a family member that worked in the field. I literally worked a few feet away from an entire analytical array of equipment and watched 100s of my own samples processes daily. I don't know dick about HPLC, FTIR, MS, GC, etc...

I'm not claiming you don't know, but I'd be sure surprised if you learned through osmosis.

Nice debate we (you and Jano) have going back and forth however.

J
yes it is good one. Although I can assure you 100% that I have not ever worked in this field, you're just going to have take my word on that one.

Just out of curiosity what kind of lab did you say your family member worked in? You seem to have good knowledge of this subject.

I can't get too specific but it is a chemical company,they have different contracts. let's say sometimes they compete with other companies too see who can take the urea from solar panels discharge and quickly and cost effectively break that down and dispose of it safely. That is just one type of contract, I got my family member a computational chemistry software so that he can practice things at home. they are not cheap the good ones are closer to 10k but I got this student version and only paid $50



@Mr_Nobody good shit brother. Lmao! I love it.
Think you could borrow that machine for a little? Lol

I've asked them multiple times but no dice, they are by the books people. It would just depend on the situation and compound maybe if I got them a fridge full of beers they would......

You seem hurt.
Bro I said don't take that seriously, I just put that anal joke their because we are getting all anal on this subject. I thought it was a clever joke, apparently not.

Your little maths part I can't really understand, because, well, it makes no sense,
That is the formula for finding area % . Let me see if I can find it somewhere online and link it for you.
hyqSnKR.png


here is the link to the pdf if you're interested,

https://www.researchgate.net/file.PostFileLoader.html?id=58d52d8e3d7f4b0de444eaea&assetKey=AS:475442940518404@1490365838782


Also I will be looking at that website you linked me , once I have time.

but right afterwards you are comparing HPLC with UV detection with GC/MS.

Does that sound like the same thing to you?
I did not compare them in the way you stated, all I said was that all software for these $100,000+ machines is the same in thr sense that they all have an internal library of a list of compounds. You cannot deny that because that would just be crazy for these companies to spend millions of dollars on their equipment sometimes 25million+ , well you can rest assured that the machine is is going have a library.
The one they use is NIST
thats the library name.

I'm saying that the kid would get slapped like a bitch if the kid thought he can shittalk a real army officer. A wonderful parallel, you should take it by the heart.

Hmmm this is taking my comparison out of context but what you said isn't wrong that kid would still be doing pushups hahahaha


too end I hope you see that formula for area% does not mean purity. also here is a kool video I thought everyone might enjoy.

 
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1. I never said purity = area. I said purity = area if normalisation approach, which is industry standard, is used.

http://www.chromatography-online.org/quant/Quantitative%20Analytical%20Methods%20for%20GC%20and%20LC/Quantitative%20%20Analysis%20Using%20Reference%20Standards/The%20Normalization%20Method.php

I did not compare them in the way you stated, all I said was that all software for these $100,000+ machines is the same in thr sense that they all have an internal library of a list of compounds. You cannot deny that because that would just be crazy for these companies to spend millions of dollars on their equipment sometimes 25million+ , well you can rest assured that the machine is is going have a library.

I can deny it perfectly.
The software for MS is completely different and the manufacturers sell it ALWAYS separately...

You don't understand what I am repeating over and over again.
The difference is hardware, those are two goddamn different machines.

Just beacause both give out graphs doesn't mean they work in any way similar.

Also, you can get older NIST libraries for 500$ and gc/ms for 3000$, but let's not get into that... A library is useless to you when you don't have mass spectrum. To get mass spectrum you need mass spectrometer. There is a mass spectrometer in GC/MS. Do you see any MS in HPLC-UV?

I will not be continuing in this discussion.
 
[QUOTE="jano, post: 1970532, member:

I will not be continuing in this discussion. [/QUOTE]


Damn. Just when I started to understand.
 
Basically just that the vial contained a substance, that was 98% pure - which is good news.

But without a standard or mass spec you cannot do identification and without a standard you cannot do mg/iu in vial.

? you did make the assumption ....
1. I never said purity = area. I said purity = area if normalisation approach, which is industry standard, is used.

http://www.chromatography-online.org/quant/Quantitative%20Analytical%20Methods%20for%20GC%20and%20LC/Quantitative%20%20Analysis%20Using%20Reference%20Standards/The%20Normalization%20Method.php



I can deny it perfectly.
The software for MS is completely different and the manufacturers sell it ALWAYS separately...

You don't understand what I am repeating over and over again.
The difference is hardware, those are two goddamn different machines.

I am only comparing the softwares in the sense that they have libraries to see the compounds nothing else, not comparing machines.....


the uv library is called spectra.
the library for perkin elmers are nist
the librarys for shimadzu machines here

Mass Spectral Libraries and Databases : SHIMADZU (Shimadzu Corporation)



they do have people who run hplc ms after hplc uv to confrim the structure of compounds
 
Anyone try PPL's Halo. I am on the fence about pulling the trigger here. I just want some feedback from members that have used PPL's Halo.
 
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