Dirts Brew log

[Spaceman Spiff]

I'm not worried about airborne to be honest.
My issue with it is the sterilization portion of bottle top filters and autoclave of septa tops.

 

[Dirthand]

I’m no expert, and by no means can answer the question you’ve asked mate sorry, but don’t we push ‘unsterile’ air into the vial everytime we use it ? I wouldn’t have though a couple of seconds of air exposure would have mattered considering the vial isn’t airtight every single time we jab a needle into the top

I wouldn't think it was a big deal either.. that's why I am trying to get some realistic experienced advice.. I just want to get a solid process down that I can trust. Really the testing will tell the tell. Honestly I think there is probly small amounts of bacteria in a lot of the stuff we inject. Our immune systems are in place to attack harmful thing introduced into out body's. Our body's are resilient.. some more than others.. I'm sure I can study up in some medical journal somewhere about acceptable levels of this and that... maybe I will.. probably not tho!!

 

[Dirthand]

I'm not worried about airborne to be honest.
My issue with it is the sterilization portion of bottle top filters and autoclave of septa tops.

The bottle top filters and media bottles I have are sterilized with gamma radiation and packed in sterilized pouches. My other media bottles and septas I dip them in bleach then wash them with dawn dish soap and rinse with distilled water then I dry heat sterilize them at 450 degrees for about 2.5 hours which is way hotter and longer than supposably needed. Is this process .worthy..? I dont really know supposably.. it took quite of bit of searching for me to find sterilized nylon .22 bottletop filters as well.another reason I started using the bottle top filter is because I just didn't trust the one rime syring filter.. this way it gets filtered twice

 

[qadsan]

Update on the bottletop filters from ks-tek.com.. finally came in. They come with media bottles all sterilized with gamma radiation . So basically it's a closed system except for the second or 2 it takes to detach the media bottle from the filter and put the cap on (I use caps with a septa so I can draw out of the media bottle as needed. I a discussion. In a thread yesterday I was told bya couple people that this is a very unsterile practice.. I guess because the short time that it is exposed to the open air switching out filter and cap. It doesn't bother me that mu h because when I do transfer I use syringe filters so it gets filtered again.. however I just dint understand what is so unsterile about that process.. if some of you experienced guys can chime in. I'd appreciate it. @narta @Heavy Iron @qadsan @DECLAN @ksm2 @Spaceman Spiff .

Ideally, you should be doing everything under a flow hood equipped with a 0.22um filter to minimize the chances of problems while using sterile gloves and mask, but not everybody has a flow hood to use. The next best thing I can think of while using sterile gloves and mask and doing this in as clean of a room as possible with no air movement is the process you’re using with a sterile reagent bottle and septum, then transferring to your sterile 10mL vials via syringe filter. Before I had a flow hood, that’s how I used to do it.

 

[Spaceman Spiff]

Ideally, you should be doing everything under a flow hood equipped with a 0.22um filter to minimize the chances of problems while using sterile gloves and mask, but not everybody has a flow hood to use. The next best thing I can think of while using sterile gloves and mask and doing this in as clean of a room as possible with no air movement is the process you’re using with a sterile reagent bottle and septum, then transferring to your sterile 10mL vials via syringe filter. Before I had a flow hood, that’s how I used to do it.

4 HEPA filter machines in a 4x 4 room. Good to go

 

[narta]

Update on the bottletop filters from ks-tek.com.. finally came in. They come with media bottles all sterilized with gamma radiation . So basically it's a closed system except for the second or 2 it takes to detach the media bottle from the filter and put the cap on (I use caps with a septa so I can draw out of the media bottle as needed. I a discussion. In a thread yesterday I was told bya couple people that this is a very unsterile practice.. I guess because the short time that it is exposed to the open air switching out filter and cap. It doesn't bother me that mu h because when I do transfer I use syringe filters so it gets filtered again.. however I just dint understand what is so unsterile about that process.. if some of you experienced guys can chime in. I'd appreciate it. @narta @Heavy Iron @qadsan @DECLAN @ksm2 @Spaceman Spiff .

Don't sweat it.

I knew major UGLs that poured into straight from the factory vials not even giving them a proper wash and dry cycle and no one got an infection. We are talking tens of thousands of vials.

Ffs we used to grind pellets, shive them through coffee filters and straight out shoot that shit and still be alright - most of the time.

What you can do to keep things as sterile as possible is make a mixture of chlorine and water like 10% put it in a spray bottle and spray the air in the are you will work on. It will somewhat clean the air from particles.

 

[McLovin.]

Don't sweat it.

I knew major UGLs that poured into straight from the factory vials not even giving them a proper wash and dry cycle and no one got an infection. We are talking tens of thousands of vials.

Ffs we used to grind pellets, shive them through coffee filters and straight out shoot that shit and still be alright - most of the time.

What you can do to keep things as sterile as possible is make a mixture of chlorine and water like 10% put it in a spray bottle and spray the air in the are you will work on. It will somewhat clean the air from particles.

its all fun and games till you get sepsis. keep everything as clean as possible no reason not to be hygienic when you're gonna be injecting the stuff

 

[narta]

its all fun and games till you get sepsis. keep everything as clean as possible no reason not to be hygienic when you're gonna be injecting the stuff

I didn't say fuck it go raw, I said don't sweat it. The only time I saw someone get an infection was from their own mistakes, me and my partner were using the same QV test he got an infection I didn't. But he did prick himself twice before doing it right so he probably picked up bacteria from his own skin

 

[Dirthand]

its all fun and games till you get sepsis. keep everything as clean as possible no reason not to be hygienic when you're gonna be injecting the stuff

I don't think he is actually promoting unsafe practice.. he is just putting things into perspective the way my country dumb self can understand

 

[McLovin.]

i understand i meant just in general if you can do simple hygienic things to keep everything safe there is no reason to not do those things ensure you dont have any incidents happening

 

[AlexDavis43]

For the bubble boyz, your favorite homebrew luchador. Flow hood, what?

 

[Dirthand]

For the bubble boyz, your favorite homebrew luchador. Flow hood, what?

i wonder what dudes up to now. Possibly a vendor here on meso?

 

[McLovin.]

i wonder what dudes up to now. Possibly a vendor here on meso?

he could be any of us we will never know

 

[Dirthand]

he could be any of us we will never know

I could never be that cool!

 

[McLovin.]

I could never be that cool!

same :c just look at me T_T

 

[Zebedee]

Don't sweat it.

I knew major UGLs that poured into straight from the factory vials not even giving them a proper wash and dry cycle and no one got an infection. We are talking tens of thousands of vials.

Ffs we used to grind pellets, shive them through coffee filters and straight out shoot that shit and still be alright - most of the time.

What you can do to keep things as sterile as possible is make a mixture of chlorine and water like 10% put it in a spray bottle and spray the air in the are you will work on. It will somewhat clean the air from particles.

I know of a popular UK lab who used to do that too & without any reports of problems arising on forums. What killed the lab was WEDINOS testing in the early-mid 2010’s showing that their products didn’t contain what they were supposed to, eg TTM blend with no mast in it etc.

I don’t think I’ve ever known anyone pushing a “fuck it, whatever …” approach to HB on here, but the total opposite paranoia & scaremongering that’s cropped up recently is ridiculous. “Airborne particles” polluting your gear in 1-2 seconds … fuckwits arguing that have clearly never drawn from a pharma amp, something that happens tens of thousands of times every day around the globe without patients getting sick from “airborne particles” in their medication

 

[DECLAN]

I know of a popular UK lab who used to do that too & without any reports of problems arising on forums. What killed the lab was WEDINOS testing in the early-mid 2010’s showing that their products didn’t contain what they were supposed to, eg TTM blend with no mast in it etc.

I don’t think I’ve ever known anyone pushing a “fuck it, whatever …” approach to HB on here, but the total opposite paranoia & scaremongering that’s cropped up recently is ridiculous. “Airborne particles” polluting your gear in 1-2 seconds … fuckwits arguing that have clearly never drawn from a pharma amp, something that happens tens of thousands of times every day around the globe without patients getting sick from “airborne particles” in their medication

Most of us here have been running ugl, hombrew and probably vet grade steroids for more/less than a decade. And I bet there’s less than 5% who have dealt with an infection much more an abscess that needs to be drained.

I think if you did all the proper hygienic injection protocol and you still get infected, then you’re just weak and it’s natural selection lol.

Bottom line, buy from a reputable source and effing don’t be a slob, be sanitary when injecting and never reuse a needle. My personal habit as a sanitary precaution is to inject as soon as I get off the shower.

 

[iris]

Little update: gonna try my biggest brew most likely this weekend.. looks like that case of bottletop filters and couple hundred syringe filters ( both nylon and sterile) will be arriving next day or so !! I will be making testosterone deconate 500 mg/ml. I will be using this ester personally for cruise/blast going forward and see how it goes. Making a bigger batch makes sense as I am curious to send in a sample to have it tested for a wide range of things.. such as sterility, microb, concentration ect.. I'm not sure what all test are available but imm gonna find out and do it.. I'm curious to know how things are coming out. Shit I might wish I didn't know once I find out!!! I do want to say that part of harm reduction is people that has been doing this a while helping people like me figure it out and there has been several guys do that.. special thanks to @narta I bug the crap outra this guy and he is always happy to answer my stupid questions.. thanks my guy

You are baking enough stuff for the whole town and doing a Readalot.
Jeez, I leave a minute and it's party time.
I should leave more often.

How are you?
M just catching up with you guys today.
You will be busy in the kitchen, so til next time.
Hope it all goes to plan.
X

 

[Dirthand]

You are baking enough stuff for the whole town and doing a Readalot.
Jeez, I leave a minute and it's party time.
I should leave more often.

How are you?
M just catching up with you guys today.
You will be busy in the kitchen, so til next time.
Hope it all goes to plan.
Xmg/ml

No Kitchen for me today. Relaxing!! Glad to hear your doing ok!

 

[Dirthand]

Well did a couple of things this weekend! Made the 250ml batch of Test D 500mg/ml and it turned out crystal clear but with a very slight yellow tint. Paid for the jano concentration test on friday so ill get that sent off on tuesday along with the sample for the sterility test. The sterility test im told will take up to 3 weeks. The filter process for the Test D too a very long time. I read more about nylon filters not being the best because the are protein binding. I read so much conflicting information. It seems that really the only way to go is PTFE. will pvdf and nylon work.... yes but from now on i will only purchase ptfe.. I keep reading about people using pes but i know from personal experience that pes will melt in a heartbeat. But yes it took about 2.5 hours for 250ml of test d 500mg/ml to filter through a nylon bottle top filter at 20psi.. very slow drip. i was very amazed at the amount of powder to liquid it takes to make 500mg/ml.. I was thinking to myself there is no freaking way im gonna get all this shit to disolve in there.
Test D 500mg/ml
BA 2%
BB 25%
MCT
Also i have been making several small batches of TNE trying to figure out what i can get to hold with the least amount of super solvent. I had labs done on 8/6 after a week of using some i made will low amounts of EO. Mt c-reactive protein was in low.. like a 1.7 . i kept playing with higher EO cause kept crashing.. just kinda playing around learning by experince. It seemed that EO did not bother me that much. Long story short I threw that bottle of shit in the trash. I ended up making a batch with high amount of EO did a little pin the day or 2 before and the mornin of bloodwork i had done on 8/26 not only did the high amount of EO swell up my delt and big red spot for a day my c-reactive protein was through the roof. No more EO for dirthand. Made some tne this weekend with castor oil and BB seems ok so far. You guys can say told you so about the EO. itys cool.. I just gotta figure it out the hard way sometimes