Janoshik Analytical laboratory testing services

[janoshik]

Hi janoshik - fellow chemist here (I work in synthetic chemistry, not analytical.). Just made an account to respond lol.

When you say a new testing method - is this a different solvent blend/column packing material? From the HPLC, it looks like you're getting much better separation of some other product (the 5.276 / 6.103 retention time products).

Any idea what those peaks are? Some sort of polymerization product or something?

Anywho, very cool!

My pleasure!

This is different stationary phase and different gradient program - so both parameters you've mentioned changed.

If you see a bit of a non-Gaussian shape on the right shoulder of the GH in the old method (around 4.05 min), that's the 5.276 peak in the new method - going from no separation to a great one! I haven't done my own research on the identity of those impurities, but Karlsson made a few really nice publications about it and the stuff I'm seeing is the same he saw during his analyses - usually just very minor modifications of the GH molecule itself.

Also, if you check his article linked below, even he didn't achieve peak shape that was perfectly Gaussian, so one can only assume that I've chromatographically separated what he did not - if I may boast a little.

Ref: Separation of Recombinant Human Growth Hormone Variants by UHPLC


Polymerization is tested in the dimer and related proteins test - which is conducted via size exclusion chromatography, as RP-HPLC ain't quite cut for that.

Cheers

 

[jwarr]

super interesting! I wonder if those products retain biological activity. If so, purity becomes more of a cerebral question vs practical one. It would be really interesting to see the purity of pharmaceutical grade products. Even in GMP facilities, we see changes in protein products over time (infliximab being the most well studied example).

 

[janoshik]

super interesting! I wonder if those products retain biological activity. If so, purity becomes more of a cerebral question vs practical one. It would be really interesting to see the purity of pharmaceutical grade products. Even in GMP facilities, we see changes in protein products over time (infliximab being the most well studied example).

I have no doubt they do, actually - the question is to what extent and what negatives do they bring? Not much research was done on that (although some was iirc).

The issue with comparing the purity of pharmaceutical grade products is that it's oft impossible to tell if it's genuine or good fake.

However, according to what I've seen over the years, there is not much difference between great generic (meaning UGL) and pharma GH.

 

[Norse13]

There are accredited testing sources instead of this shady ass mother fucker and you, who come here claiming to give a shit about your wife... choose to spend less on testing shit that you are putting inside of HER body instead of paying a bit more for an accredited testing source to test it? You choose to take a discount at the cost of your wife's health? Shame on you.

Get the fuck outta here clown.

In that case, good sir, could you list some of these accredited labs that will test unlabelled anonymous drugs, paid anonymously with bitcoin?
What are their charges?
Have you yourself sent any steroids for testing to these labs?

 

[BodyMechanic]

In that case, good sir, could you list some of these accredited labs that will test unlabelled anonymous drugs, paid anonymously with bitcoin?
What are their charges?
Have you yourself sent any steroids for testing to these labs?

You were dumb enough to stumble in here right? Look em up Nurse!

 

[janoshik]

You were dumb enough to stumble in here right? Look em up Nurse!

There are none, tho.

Chemtox and Simec don't accept blind samples at all and Lab4Tox doesn't accept BTC.

 

[Norse13]

You were dumb enough to stumble in here right? Look em up Nurse!

I take it that you have never actually used any...

 

[BodyMechanic]

I take it that you have never actually used any...

I've used Jano and Lab4 for the same samples before. I stuck with Jano because I have sent him samples with really odd dosages and he's been spot on. There is zero way he could know otherwise without HPLC. Unless he's got a high end spectrometer and he's autistic with random algorithms reading those somehow lol

I'm just trolling bro don't pay me no mind.

 

[janoshik]

No one ever asked to have it removed. I planned on removing it years and years ago. But one too many snarky remarks made it less of a priority.

Thank you.

 

[jwarr]

Janoshik - can you test capsules (anavar specifically)? I'm assuming its anavar + excipients like starch/stearic acid. If so, what is the price?

 

[janoshik]

Janoshik - can you test capsules (anavar specifically)? I'm assuming its anavar + excipients like starch/stearic acid. If so, what is the price?

Sure, no problem - common AAS capsules are 80 Euro per sample.

 

[jJjburton]

Have you upgraded to that better testing method yet?

While the new method do hgh and steroids?

 

[janoshik]

Have you upgraded to that better testing method yet?

While the new method do hgh and steroids?

The new GH testing method will be used starting 2021.

 

[Norse13]

@janoshik Question for the chemist here:
There's a lot of Internet ramblings of good vs bad Primo (and Var).
But what's the difference, if something HPLC tests as primobolan, is there really any difference between pharma and UGL? If it tests the same, then what's the difference??

 

[janoshik]

@janoshik Question for the chemist here:
There's a lot of Internet ramblings of good vs bad Primo (and Var).
But what's the difference, if something HPLC tests as primobolan, is there really any difference between pharma and UGL? If it tests the same, then what's the difference??

If it tests as primo and / or var then there's no difference as far as I know.

There can be mild differences with var pills, but not because of the compound, but because of pill quality.

Many underground manufacturers press VERY hard pills, that take much longer to dissolve in stomach, so for example the pre-workout 'edge' - which is what many people tend to go by, simply isn't there as it's delayed or spread over a but longer time.

 

[Norse13]

If it tests as primo and / or var then there's no difference as far as I know.

There can be mild differences with var pills, but not because of the compound, but because of pill quality.

Many underground manufacturers press VERY hard pills, that take much longer to dissolve in stomach, so for example the pre-workout 'edge' - which is what many people tend to go by, simply isn't there as it's delayed or spread over a but longer time.

Thanks for clarification - with my basic biology & chemistry (& engineering) I also don't see meaningful differences. It's 2020, time to put down this Internet myth to death now. "Dbol (insert name of any steroid) was much more potent in the 1980s and 1990s..."

 

[janoshik]

Thanks for clarification - with my basic biology & chemistry (& engineering) I also don't see meaningful differences. It's 2020, time to put down this Internet myth to death now. "Dbol (insert name of any steroid) was much more potent in the 1980s and 1990s..."

Well, I assume during the entire 80 and 90s less actual tests of the gear were conducted than there are done in a month now, so it's hard to know what the state of things was back then.

It's that along with mix of nostalgy, but that's just my personal opinion. You know, nostalgy like the grass was greener when I was younger too

 

[ElFlaco]

@janoshik Hi! Im from spain. I like to send you some samples of raw steroids. How can I contact you? Is there a problem from my country? How much does each analysis cost?

 

[janoshik]

@janoshik Hi! Im from spain. I like to send you some samples of raw steroids. How can I contact you? Is there a problem from my country? How much does each analysis cost?

Hello,

please, email me at info [at] janoshik.com

No problem, I have a fair amount of clients from Spain.

Cost for common anabolic steroid analysis is 80 Euro per sample.

Cheers

 

[jwarr]

@janoshik - are you able to do an HLPC separation/quantification of Dimethylnandrolone Undecanoate? It has a number of stereocenters and I'd be interested in specific enantiomeric purity.